ngwaahịa

Nwamba.KB01009K-50T

Ihe gbasara
A na-eji ngwa a maka nyocha qualitative ngwa ngwa nke tetracyclines na nlele mmanụ aṅụ.

Usoro nkwadebe sample
(1) Ọ bụrụ na mmanụ aṅụ sample crystallized, kpoo ya na mmiri ịsa ahụ adịghị elu karịa 60 ℃, ruo mgbe mmanụ aṅụ sample mebie, mix kpamkpam, jụrụ dị ka ụlọ okpomọkụ, mgbe ahụ arọ maka assay.
(2) tụọ 1.0 ± 0.05g homogenate n'ime 10ml polystyrene centrifuge tube, tinye 3ml sample mmịpụta ngwọta, vortex maka 2min ma ọ bụ ịkwanye ya n'aka ruo mgbe sample-agwakọta kpamkpam.

Arụ ọrụ nyocha.
(1.) Were karama dị mkpa site na ngwugwu kit, wepụ kaadị achọrọ, ma mee akara kwesịrị ekwesị.Biko jiri kaadị ule ndị a n'ime 1h mgbe emepechara ngwugwu.
(2.) Were 100l kwadebere ihe nlele n'ime oghere nlele site na pipette, wee malite ngụ oge mgbe mmiri na-asọ.
(3.) Tinye maka 10min na ụlọ okpomọkụ.
7.LOD

Nsonaazụ
Enwere ahịrị abụọ na mpaghara nsonaazụ kaadị, akara njikwa na Tetracylicine Line, nke edobere nkenke dị ka “B” na “T”.Nsonaazụ ule ga-adabere na agba nke ahịrị ndị a.Eserese na-esonụ na-akọwa njirimara nsonaazụ.
Na-adịghị mma: akara njikwa na ule ule abụọ na-acha uhie uhie na T Line dị ọchịchịrị karịa akara njikwa;
Tetracyclines Positive: akara njikwa na-acha ọbara ọbara, T Line enweghị agba ma ọ bụ T Line dị ọkụ karịa ahịrị C, ma ọ bụ T Line bụ otu C Line.

Nchekwa
2-30 Celsius C n'ebe gbara ọchịchịrị, anaghị ajụ oyi.Ngwa ahụ ga-adị irè n'ime ọnwa iri na abụọ.A na-ebipụta nọmba nza na ụbọchị kubie ume na ngwugwu ahụ.